Immersive Translation - Bilingual Document Translation: One-Click Translation of PDF, ePub eBooks, Subtitle Files, txt Files

Number:VR—VM/RD—2—1210—00 12 / 47

GuangzhouWanfu Biotechnology Co., Ltd.

Number:VR—VM/RD—2—1210—00

Fully AutomaticImmunohistochemical Staining MachinePA-3600

Verification Report

Report Development

Hardware and New Technology Department / Prepared by: Date:

Report Review

Hardware and New Technology Department / Reviewer: Date:

Instrument Development Department 1 / Reviewer: Date:

Report Approved

Total person in charge of company verification: Date:

Number:VR—VM/RD—2—1120—00 13 / 47

Table of Contents

Directory 1

Chapter 1: Overview 2

1.1 Verification Purpose 2

1.2 Test basis 2

1.3 Test Information 2

1.3.1 Instrument Use Environment 2

1.3.2 Prototype 2

1.3.3 Test Equipment 2

1.4 Test Results 2

1.4.1 Test Results 2

Chapter 2: Test Records 5

2.1 Appearance and Structure Verification 5

2.2 Instrument Function Verification 5

2.3 Basic Performance 7

2.3.1 Temperature Accuracy of the Staining Module Heating Component 7

2.3.2 Temperature Fluctuation of the Staining Module Heating Component 11

2.3.3 Reagent Addition Accuracy 14

2.3.4 Reagent Addition Volume Repeatability 16

2.4 Network Security Verification 17

2.4.1 Data Interface 17

2.4.2 User Access Control 18

2.5 Climate Environment Testing 19

Chapter 1: Overview

1.1 Verification Purpose

Fornewly developedfully automated immunohistochemical staining machinePA-3600developmentprototype stagetoappearance, performanceand climate environment adaptability to be verifiedevaluation.

1.2 Test Basis

《Fully Automated Immunohistochemical Staining Machine PA-3600Test Plan》Document Number:VS—VM/RD—1—1210—00

1.3 Test information

1.3.1 Instrument usage environment

1）Operating temperature: 18℃～30 ℃；

2) Relative humidity: 20%～70% (no condensation);

3) Atmospheric pressure:Less than2000m;

4) Rated voltage:AC100～240V;

5）Frequency ：50Hz/60Hz；

6) Avoid direct exposure to strong light;

7) Use in a well-grounded environment and avoid dust, acidic volatile gases, vibration and strong electromagnetic interference.

1.3.2 Prototype

The sample numbers are:PA36002009100001(referred to as 1#)

1.3.3 Test Equipment

Equipment Calibration Number	Test equipment	Model Number	Calibration date	Validity Period
08-53	Data acquisition instrument	DAQ970A	2024-03-28	One year
/	Analytical Balance	ME104E	2024-07-08	One year
/	Hydrometer	DENSITO	2024-03-28	One year
/	Thermohygrometer	/	2024-03-28	One year
/	Walk-in Temperature and Humidity Test Chamber	/	2024-03-28	One year

1.4 Test Results

Test Results 1.4.1

Serial Number	Test Project	Test function phenomenon	Result Judgment	Notes
	Appearance structure	The prototype appearance should be clean, free of cracks, burrs or scratches, and the text and markings on the panel should be clear and accurate 2) The prototype's moving parts operate smoothly without any jamming or sudden jumps. The operation is stable and there is no significant noise 3) Fastener connections shall be strong and reliable, with no looseness	合格	/
	Functionality testing	1）Used for sample processing before pathological analysis, fully automated completion of dewaxing, antigen retrieval, staining and fluorescence in situ hybridization (FISH) detection pretreatment, post-hybridization washing; 2）The fully automated immunohistochemical staining machine has 36 staining sites; 3）The fully automated immunohistochemical staining machine has 40 reagent positions; 4）Equipped with slide QR code scanning system; 5）The software interface can display the buffer volume in real time; 6）Has the function of classifying and storing waste liquid; 7）has a patient information management system; 8）Has reagent information management system	合格	/
	Dyeing Module Heating ComponentTemperatureAccuracy	At18℃、24℃、30℃、30℃/70%RHenvironment, the dyeing module meets the requirements at the temperature control points (37℃, 65℃, 85℃, 101℃): the maximum (MAX) and minimum (MIN) of the stable internal temperature should be within the range of T±2℃.	合格	/
	Dyeing module heating componenttemperaturefluctuation	Operating under conditions of 18℃, 24℃, 30℃, and 30℃/70%RH, the staining module meets the requirements at all temperature control points (37℃, 65℃, 85℃, 101℃): Temperature fluctuation < 0.5℃.	合格	/
	Reagent Addition Accuracy	Reagent addition volume control points (100μL, 130μL, 240μL, 370μL, 500μL)all meet:addition volume deviation P_v≤3%.	合格	/
	Reagent Addition Repeatability	Reagent addition volume control points (100μL, 130μL, 240μL, 370μL, 500μL)all meet: repeatability CV ≤ 5%.	合格	/
	Data Interface	Dyeing machine data interface includes network interface and serialinterface, where the network interface is used to realize communication between the dyeing machine and the computer, serialinterfaceisused by the manufacturer to debug the instrument, users can't use it. 1) Wired networks primarily use manufacturer-defined protocols for transmission. 2) Serial interface: Transmission protocol: RS232; Data format: Baud rate 115200,no parityevenparity, no TS/CTS, 8data bits, nostop bit.	合格	/
	User Access Control	User types: regular user, administrator user User Authentication: Upon boot-up, user permissions are determined based on the defined username. After entering the software, functionalities are restricted or opened based on the user's permissions Regular users can perform dyeing operations; administrator users can perform dyeing operations and can add or delete regular user accounts.	合格	/
	Low-temperature test at rated operating conditions	Prototypestructureappearance, temperature control, sample loading performance, and functionality all meet the technical requirements.	合格	/
	High temperature test for rated operating conditions	Mockupstructureappearance, temperature control, sample loading performance and functionality all meet the technical requirements.	合格	/
	Rated operating humid heat test	Prototypestructureappearance, temperature control, sample loading performance and function all meet the technical requirements.	合格	/
	Low-temperature storage test	After the testprototypestructureappearancetemperature control, sample loading performance and functions all meet the technical requirements.	合格	/
	High temperature storage test	After the testprototypestructureappearancetemperature control, sample loading performance and function all meet the technical requirements.	合格	/
	Damp Heat Storage Test	Aftertheprototypestructureappearance、temperaturecontrol、sampleadditionperformanceandfunctionallmeetthetechnicalrequirements。	合格	/

Number:VR—VM/RD—2—1120—00 16 / 23

Chapter Two: Test Records

2.1 Appearance and StructureVerification

2.1.1 Test Information

Mockup Quantity: 3 units Mockup Number: 1#、2#、3#

Testing Tool: Visual Inspection Tester: Yang Qingyun

2.1.2 Test Method

Inspect3prototype units, visually inspect the appearance, structure, keys, fastener connections, etc. of the units in natural light with normal or corrected vision, and confirm whether the appearance and structure meet the design requirements.

2.1.3 Judgment Criteria

1) The appearance should be clean and free of cracks, burrs or scratches, and the text and markings on the panel should be clear and accurate;

2) Fully automated immunohistochemical staining machineMoving parts should be smooth, without jamming or jumping, working smoothly and without significant noise;

3) Fastener connections should be strong and reliable, and should not be loose.

2.1.4 Test Results

The prototype appearance should be clean, free of cracks, burrs or scratches, and the text and markings on the panel should be clear and accurate

2) The prototype's moving parts operate smoothly without any jamming or sudden jumps. The operation is stable and there is no significant noise

3) Fastener connections should be strong and reliable, without looseness.

2.2 Instrument Function Verification

2.1.1 Test Information

Mockup quantity: 3 units Mockup number: 1#, 2#, 3#

Test Tool: Visual Inspection Tester: Yang Qingyun

2.1.2 Test Method

Check3prototype units and system software functionality, perform full-process testing according to the following requirements:

Visually check if the fully automated immunohistochemical staining machine has36positionstomeet the design requirements.

Visually inspect and manually install the reagent rack, confirming the number of reagent bottles that can be installed

Is there a QR code scanning system visible?

Visually inspect the software interface for the buffer bottle options

Visually assess whether there are two categories for storing waste liquid drums;

Detect whether the software interface has a patient information management system;

7) Check whether the software interface has a reagent information management system.

2.1.3 Judgment Criteria

1）It can be used for sample processing before pathological analysis, fully automatically completing including dewaxing, antigen retrieval, staining, and fluorescence in situ hybridization (FISH) detection pretreatment, post-hybridization washing;

2）The fully automated immunohistochemical staining machine has 36 staining positions;

3) The fully automated immunohistochemical staining machine has 40 reagent positions;

4）Equipped with slide QR code scanning system;

5）The software interface can display the buffer volume in real time;

6）has the function of classifying and storing waste liquid;

7）Has a patient information management system;

8) Possesses a reagent information management system.

2.1.4 Test Results

1）Can be used for sample processing before pathological analysis, automatically completing the whole process including dewaxing, antigen repair, staining and fluorescence in situ hybridization (FISH) detection pretreatment, hybridization after washing.

2）The fully automated immunohistochemical staining machine has 36 staining positions;

3）The fully automated immunohistochemical staining machine has 40 reagent positions;

4）equipped with slide QR code scanning system;

5）The software interface can display the buffer volume in real time;

6）Has the function of classifying and storing waste liquid;

7）Has a patient information management system;

8) Has a reagent information management system.

2.1.5 Test data

Number:VR—VM/RD—2—1120—00 16 / 23

2.3 Basic Performance

2.3.1 Dyeing Module Heating ComponentsTemperatureAccuracy

2.3.1.1 Test Information

Mockup quantity: 1 unit Mockup number: 1#

Testing tools:DAQ970A data acquisition instrument Tester: Yang Qingyun

2.3.1.2 Test Method

Use a temperature measuring slide (with a temperature probe fixed) to test according to the following method at ambient temperatures of 18℃, 24℃, 30℃, and 30℃/70%RH:

The lower machine controls 36modules to heat up together. Prepare the test slides before testing. The working area of the slide is fixed1NTC temperature sensor. The NTC should be as close to the upper surface as possible to ensure the surface of the slide is flat.

Turn on the sample stage, place the slide in place and control the cover slip according to table2.3.1.2.1to press down to the preset position and reset, then select the preset position for the cover slip to press down according to different test temperature points and control the heating element to reach the temperature control point T corresponding to the preset position.

③ After the temperature stabilizes (please check the temperature stabilization time table for the stabilization time of each temperature point), collect data at an interval of 1S for a total time of 5min. Select the maximum value (MAX) and minimum value (MIN) of the temperature within the temperature stabilization period, respectively.

Table2.3.1.2.1 Preset Location and Temperature Control Point Correspondence Table:

Temperature Set Point (°C)	Press down the cover to the preset position	Stabilization Time (min)
37	A7	3
65	A4	5
85	A3	8
101	A3	10

2.3.1.3 Judgment Criteria

At environmental temperatures of18℃, 24℃, 30℃, the staining module requires that at temperature control points T of 37℃, 65℃, 85℃, and 101℃, the NTC temperature of all data points (maximum (MAX) and minimum (MIN) temperature within the stable temperature) of the temperature sensor glass in the reaction zone should be within the range of T±2℃.

2.3.1.4 Test Results

Test results all meet 2.3.1.3judgment criteria requirements, specific data see 2.3.1.5.

2.3.1.5 Test Data

2.3.1.5.1Test data:

Table 2.3.1.5.1

37℃ setpoint stable temperature (unit/℃)
Module Number	18°C Low Temperature		Room temperature environment 24℃		High temperature environment 30℃		30℃/70%RHEnvironment
	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius
0#	37.23	37.06	37.30	37.07	37.20	36.96	37.26	36.97
1#	37.30	36.98	37.11	37.00	37.28	37.02	37.28	36.91
2#	37.21	36.91	37.23	37.04	37.27	36.95	37.19	37.02
3#	37.26	36.97	37.24	37.00	37.11	36.94	37.27	37.06
4#	37.13	36.97	37.27	37.01	37.28	37.05	37.11	37.04
5#	37.15	37.08	37.28	36.91	37.27	37.01	37.16	37.08
6#	37.29	37.03	37.21	37.03	37.30	36.94	37.22	36.94
7#	37.14	36.92	37.22	36.97	37.16	37.00	37.28	37.03
8#	37.10	36.96	37.26	37.08	37.15	36.99	37.11	36.90
9#	37.24	36.90	37.20	37.02	37.14	37.06	37.21	36.97
10#	37.25	37.01	37.20	37.04	37.21	36.96	37.18	36.95
11#	37.26	36.94	37.24	37.05	37.26	37.01	37.19	36.90
12#	37.14	37.01	37.24	37.09	37.26	36.98	37.17	36.97
13#	37.21	36.90	37.12	36.98	37.29	37.07	37.30	37.00
14#	37.22	36.91	37.11	36.93	37.17	37.07	37.16	36.91
15#	37.13	36.96	37.11	36.96	37.16	37.01	37.12	36.91
16#	37.26	37.06	37.11	36.94	37.26	37.01	37.20	37.02
17#	37.28	37.05	37.23	37.04	37.28	36.98	37.17	36.96
18#	37.25	36.90	37.28	37.01	37.25	36.95	37.29	36.91
19#	37.15	37.05	37.20	37.08	37.16	36.90	37.17	37.01
20#	37.16	37.03	37.11	37.05	37.30	37.06	37.18	37.02
21#	37.12	36.93	37.22	36.94	37.25	37.05	37.19	37.09
22#	37.13	36.92	37.21	36.91	37.10	36.93	37.14	37.06
23#	37.26	37.06	37.12	36.97	37.10	36.94	37.22	37.09
24#	37.11	37.00	37.29	37.05	37.26	36.92	37.30	36.92
25#	37.12	37.00	37.23	37.09	37.30	37.07	37.28	36.92
26#	37.20	36.90	37.10	36.92	37.17	37.00	37.25	36.98
27#	37.17	37.02	37.15	37.09	37.22	37.01	37.24	37.08
28#	37.10	37.03	37.28	37.07	37.28	36.95	37.24	37.03
29#	37.29	36.98	37.17	37.09	37.26	36.97	37.13	36.92
30#	37.30	36.93	37.11	36.93	37.19	37.07	37.23	36.95
31#	37.15	36.99	37.26	37.05	37.16	36.91	37.18	37.08
32#	37.19	37.09	37.19	36.96	37.16	37.06	37.27	36.90
33#	37.14	36.91	37.26	36.93	37.15	36.94	37.23	37.03
34#	37.15	37.08	37.17	37.04	37.20	37.08	37.24	36.98
35#	37.12	36.91	37.30	37.01	37.15	36.90	37.11	36.96

Table 2.3.1.5.2

65℃ temperature control point stable temperature (unit/℃)
Module Number	18°C Low Temperature		Room temperature environment 24℃		High temperature environment 30℃		30℃/70%RHenvironment
Module Number		MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius
0#	65.15	64.76	65.10	64.87	65.10	64.72	65.08	64.74
1#	65.00	64.87	65.23	64.90	65.26	64.75	65.09	64.89
2#	65.21	64.81	65.14	64.74	65.04	64.73	65.04	64.78
3#	65.00	64.80	65.11	64.71	65.14	64.75	65.25	64.89
4#	65.00	64.85	65.14	64.81	65.13	64.87	65.09	64.86
5#	65.26	64.76	65.27	64.89	65.11	64.88	65.24	64.86
6#	65.20	64.79	65.23	64.83	65.24	64.73	65.30	64.86
7#	65.15	64.76	65.07	64.76	65.07	64.82	65.21	64.87
8#	65.17	64.74	65.14	64.89	65.10	64.90	65.25	64.77
9#	65.08	64.83	65.26	64.85	65.12	64.84	65.04	64.85
10#	65.20	64.76	65.10	64.73	65.06	64.74	65.29	64.84
11#	65.12	64.87	65.28	64.79	65.16	64.78	65.02	64.70
12#	65.30	64.70	65.10	64.80	65.26	64.73	65.00	64.83
13#	65.27	64.77	65.23	64.84	65.20	64.71	65.12	64.70
14#	65.20	64.85	65.18	64.76	65.15	64.75	65.26	64.70
15#	65.30	64.72	65.27	64.84	65.04	64.71	65.04	64.72
16#	65.15	64.75	65.28	64.86	65.11	64.82	65.04	64.74
17#	65.02	64.86	65.24	64.87	65.27	64.84	65.13	64.74
18#	65.18	64.76	65.20	64.81	65.24	64.79	65.27	64.90
19#	65.17	64.83	65.05	64.76	65.01	64.70	65.16	64.79
20#	65.17	64.86	65.29	64.73	65.09	64.76	65.17	64.73
21#	65.18	64.83	65.13	64.73	65.28	64.81	65.09	64.76
22#	65.22	64.74	65.02	64.72	65.08	64.74	65.09	64.73
23#	65.29	64.71	65.16	64.88	65.30	64.71	65.30	64.81
24#	65.16	64.78	65.27	64.86	65.27	64.80	65.10	64.86
25#	65.05	64.77	65.29	64.89	65.16	64.82	65.14	64.82
26#	65.30	64.84	65.26	64.78	65.16	64.70	65.28	64.89
27#	65.17	64.75	65.07	64.75	65.02	64.81	65.13	64.78
28#	65.04	64.82	65.24	64.70	65.08	64.76	65.15	64.83
29#	65.00	64.80	65.14	64.83	65.01	64.75	65.27	64.81
30#	65.24	64.74	65.29	64.90	65.11	64.73	65.20	64.72
31#	65.21	64.86	65.03	64.81	65.10	64.88	65.00	64.82
32#	65.04	64.81	65.19	64.87	65.05	64.84	65.18	64.75
33#	65.17	64.82	65.25	64.78	65.27	64.81	65.08	64.74
34#	65.18	64.80	65.19	64.81	65.27	64.71	65.26	64.76
35#	65.04	64.81	65.30	64.75	65.24	64.88	65.07	64.84

Table 2.3.1.5.3

85℃ Temperature control point, stable temperature (unit/℃)
Module Number	18°C Low Temperature		Room temperature environment 24℃		High temperature environment 30℃		30℃/70%RHenvironment
Module Number		MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius
0#	85.28	84.63	85.10	84.74	85.15	84.61	85.29	84.76
1#	85.10	84.78	85.27	84.64	85.18	84.64	85.05	84.66
2#	85.29	84.69	85.15	84.65	85.03	84.74	85.04	84.78
3#	85.11	84.61	85.26	84.75	85.06	84.74	85.15	84.60
4#	85.14	84.72	85.00	84.80	85.22	84.79	85.03	84.74
5#	85.21	84.61	85.26	84.77	85.20	84.66	85.21	84.62
6#	85.12	84.72	85.29	84.66	85.28	84.65	85.13	84.63
7#	85.11	84.69	85.10	84.64	85.19	84.69	85.01	84.66
8#	85.21	84.69	85.26	84.80	85.25	84.65	85.00	84.66
9#	85.10	84.78	85.01	84.73	85.09	84.76	85.19	84.66
10#	85.02	84.75	85.02	84.64	85.11	84.71	85.07	84.66
11#	85.10	84.60	85.14	84.67	85.05	84.65	85.22	84.71
12#	85.24	84.67	85.02	84.80	85.17	84.60	85.08	84.71
13#	85.30	84.65	85.17	84.66	85.17	84.79	85.08	84.67
14#	85.16	84.68	85.13	84.74	85.14	84.62	85.19	84.71
15#	85.20	84.60	85.10	84.67	85.28	84.65	85.25	84.73
16#	85.23	84.71	85.23	84.60	85.24	84.70	85.19	84.75
17#	85.10	84.68	85.07	84.64	85.27	84.69	85.13	84.77
18#	85.22	84.63	85.02	84.66	85.25	84.79	85.07	84.61
19#	85.10	84.79	85.02	84.64	85.26	84.66	85.07	84.65
20#	85.08	84.80	85.19	84.72	85.24	84.78	85.22	84.68
21#	85.21	84.70	85.11	84.64	85.27	84.73	85.30	84.79
22#	85.03	84.73	85.03	84.69	85.01	84.77	85.07	84.73
23#	85.08	84.66	85.21	84.63	85.27	84.75	85.30	84.80
24#	85.01	84.60	85.21	84.79	85.12	84.75	85.26	84.61
25#	85.27	84.62	85.19	84.76	85.21	84.60	85.04	84.76
26#	85.00	84.66	85.27	84.75	85.29	84.75	85.01	84.65
27#	85.20	84.66	85.21	84.64	85.05	84.72	85.10	84.64
28#	85.12	84.78	85.23	84.66	85.21	84.60	85.30	84.80
29#	85.18	84.75	85.28	84.77	85.28	84.73	85.22	84.67
30#	85.29	84.69	85.14	84.69	85.15	84.75	85.03	84.79
31#	85.01	84.60	85.11	84.68	85.17	84.66	85.05	84.65
32#	85.19	84.60	85.00	84.78	85.09	84.69	85.25	84.71
33#	85.19	84.73	85.24	84.74	85.29	84.60	85.21	84.74
34#	85.07	84.62	85.24	84.65	85.20	84.60	85.09	84.64
35#	85.10	84.67	85.12	84.66	85.16	84.78	85.02	84.60

Table 2.3.1.5.4

101℃ Control Point Stable Temperature (Unit/℃)
Module Number	18°C Low Temperature		Room temperature environment 24℃		High temperature environment 30℃		30℃/70%RHenvironment
	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius	MAX/°C	Minimum / Degrees Celsius
0#	100.96	100.34	100.99	100.65	101.00	100.49	100.96	100.45
1#	100.99	100.09	100.94	100.42	100.91	100.34	100.91	100.46
2#	100.72	100.03	100.97	100.62	100.97	100.41	100.98	100.26
3#	100.64	100.01	100.93	100.25	100.93	100.68	100.99	100.40
4#	100.90	100.16	100.96	100.69	100.99	100.48	100.96	100.55
5#	100.99	100.07	100.92	100.36	100.93	100.47	100.97	100.33
6#	100.56	100.08	100.99	100.36	100.92	100.56	100.99	100.37
7#	100.96	100.06	100.90	100.42	100.91	100.53	100.92	100.47
8#	100.88	100.16	100.90	100.40	100.99	100.35	100.92	100.58
9#	100.69	100.05	100.92	100.69	100.90	100.21	101.00	100.41
10#	100.65	100.00	100.96	100.43	100.98	100.47	100.94	100.43
11#	100.94	100.32	100.94	100.60	100.97	100.27	100.90	100.56
12#	100.51	100.01	100.99	100.60	100.94	100.68	101.00	100.49
13#	100.81	99.99	100.95	100.38	100.93	100.43	100.96	100.35
14#	100.82	100.24	101.00	100.57	100.93	100.46	100.98	100.41
15#	100.81	100.04	100.98	100.35	100.91	100.50	101.00	100.62
16#	100.56	100.12	100.95	100.39	100.92	100.31	100.96	100.51
17#	100.82	100.28	100.90	100.24	100.94	100.65	100.95	100.30
18#	100.86	100.21	100.94	100.60	100.99	100.69	100.95	100.67
19#	100.57	100.31	100.95	100.24	100.92	100.23	100.93	100.28
20#	100.75	100.33	100.96	100.63	100.97	100.58	101.00	100.26
21#	100.58	100.29	100.97	100.55	100.97	100.33	100.99	100.36
22#	100.66	100.34	100.96	100.20	100.90	100.65	100.91	100.55
23#	101.00	100.12	100.90	100.25	100.98	100.21	100.96	100.30
24#	100.81	100.15	100.99	100.42	100.90	100.37	100.92	100.49
25#	100.90	100.11	100.95	100.27	100.97	100.65	100.98	100.24
26#	100.53	100.28	100.95	100.38	100.93	100.58	100.90	100.44
27#	100.86	100.33	100.95	100.48	100.91	100.64	100.95	100.45
28#	100.64	100.06	100.91	100.32	100.94	100.35	100.98	100.59
29#	100.92	100.09	100.90	100.39	100.95	100.49	100.98	100.33
30#	101.00	100.20	100.93	100.32	100.93	100.52	101.00	100.59
31#	100.78	100.14	100.96	100.27	101.00	100.56	100.94	100.24
32#	100.59	100.25	100.95	100.20	100.96	100.48	100.90	100.34
33#	100.83	100.31	100.99	100.68	100.92	100.39	100.94	100.20
34#	100.67	100.29	100.95