Lack of sensitivity (low signal-to-noise ratio) 灵敏度不足(信噪比低)
Phototoxicity & other challenges in performing living specimen experiments 进行活体实验中的光毒性及其他挑战
Need to screen a lot of organoids 需要筛选大量的器官样本
Lack of expertise in analysis or processing tools 缺乏分析或处理工具方面的专业知识
Differing protein expression levels from constructs, requiring a detector with high dynamic range 来自构建物的蛋白表达水平不同,需要具有高动态范围的探测器
THUNDER Computáional Clearing 计算清除
is an opto-digital method that removes out-of-focus blur in real-time > is fully integrated into the system's data stream 一种光数字方法,可以实时消除焦外模糊 > 完全集成到系统的数据流中
automatically takes all relevant optical parameters into account 自动考虑所有相关的光学参数
Our Solution 我们的解决方案
Imaging Organoids/Spheroids 映射器官样体/球体
Time to Result 结果时间
Rapid acquisition of blur-free images, even deep within thick samples 快速获取无模糊图像,即使在厚样本深处
Extended Range of Applications Fast overviews of whole tissue sections 应用范围扩大,快速查看整个组织切片
Higher Relevance 更高相关性
Imaging and analyzing even 对甚至进行成像和分析
challenging tissue sections with an easy workflow 具有挑战性的组织切片,采用简单的工作流程
Imaging organoids with THUNDER 使用THUNDER成像器官样体
Uncleared human brain organoid (4 colors); Image courtesy by Attria Kavyanifar. M.Sc. (supervised by Prof. Dr. Lie, Prof. Dr. Wiener) University Clinic Erlangen, Germany 未清理的人类大脑器官样体(4 种颜色);图片由 Attria Kavyanifar 提供。M.Sc.(在 Lie 教授和 Wiener 教授指导下)德国埃尔朗根大学医学院
Extend the Range of Applications to Thicker Samples 将应用范围扩展到更厚的样本
Tumor Spheroids Neurospheres
Widefield 宽场
THUNDER Imager THUNDER成像仪
Increase Relevance: Your Entry to 3D Imaging 提高相关性:您的 3D 成像入口
THUNDER Imager Cell is your versatile entry-level imaging system for a wide range of samples THUNDER成像细胞是您多样化的入门级成像系统,适用于各种样本
Monolayer Cell Culture 单层细胞培养
3D Cell Culture 3D 细胞培养
Organoids 器官样结构
Spheroids 球体
Reduce Time to Result: Upscaling Your Discovery 缩短结果时间:提升您的发现
THUNDER Imager Cell is your imaging system for a wide range of challenging 3D samples like: THUNDERImager Cell 是您用于各种具有挑战性的 3D 样本的成像系统,例如:
Spheroids such as tumoroids or neurospheres 瘤球或神经球等球体
Brain or intestine organoids 大脑或肠道器官样本
Also ideal for advanced cell culture experiments such as: 也适用于高级细胞培养实验,如:
Live cell explants or vibratome sections
Advanced applications like TIRF, FRET
Perform high resolution tissue imaging on thick samples without struggling to find your region of interest.
The recently released THUNDER Live tool visualizes a computationally cleared image instantly in the live viewer and allows you to optimize ICC parameters by using live image feedback.
Your benefits with THUNDER Live:
Get computationally cleared images directly in your live preview.
Reduce the time to optimal results with immediate visual feedback.
Intuitively and quickly find the best THUNDER ICC parameters on the fly while still in live view.
Optimize your image in one simple step without changing any settings.
THUNDER Live's blur-free view also makes selecting significant regions of interest in your sample a breeze, even on thick specimens. THUNDER Live 的清晰视野使得即使在厚样本上,选择感兴趣的重要区域也变得轻而易举。
Application example: Tumor Spheroid + THUNDER Live 应用示例:肿瘤球体+THUNDER Live
THUNDER Live reduces the time to search for biological processes in deeper regions of your living sample. THUNDER Live 减少了在活体样本的深层区域搜索生物过程所需的时间。
Focusing through a thick T47D breast cancer tumor spheroid using THUNDER Live Histone 2B GFP Objective: 20X/0.7 使用THUNDER Live Histone 2B GFP 在 厚的 T47D 乳腺癌肿瘤球体中进行对焦。目标:20X/0.7
Sample Courtesy: 样品提供:
Dr. Nariman Ansari, Leica 纳里曼·安萨里博士,莱卡
Kinetix
High End scMos 高端 scMos
camera 相机
K8
K5
Leica LEDs 莱卡 LED 灯
& Synapse & 突触
Attribute 属性
K8
Sensitivity 灵敏度
+++
++++
QE
Read Noise 读出噪声
Frame rate 帧率
40 fps 40 帧每秒
95 fps 95 帧每秒
动态范围
Dynamic
range
Cooling 冷却
No 否
Pixel size 像素大小
Resolution 分辨率
4.2 mp 4.2 百万像素
4.2 mp 4.2 百万像素
Applications 应用
使用具有非常高动态范围的固定样本进行成像。使用明亮样本进行活细胞成像。
Imaging/Thunder fixed samples
with very high dynamic range .
Live cell imaging with bright
samples.
活细胞成像低光照Thunder高速成像
Live cell imaging low light
Thunder high speed imaging
Introducing the Leica K8 scientific CMOS camera 推出 Leica K8 科学 CMOS 相机
Sensor type 传感器类型
Scientific CMOS 科学型 CMOS
Sensor size (diagonal) 传感器尺寸(对角线)
18.8 mm 18.8 毫米
Sensor Format 传感器格式
(4.2 Megapixel) (420 万像素)
Pixel dimensions 像素尺寸
Shutter mode 快门模式
Rolling shutter 滚动快门
Data interface 数据接口
USB 3 Gen2
Mechanical interface
C -mount
Exposure range
Frames per second
95 fps (in 11 bit mode)
Triggering
Yes, MMCX
Sensor cooling
at ambient
Peak quantum effciency
Dark current
pixel/second
12 Bit 1.0 e- (Median) 1.1 e-
(RMS)
16 bit 1.6 e- (Median) 1.8 e-
(RMS)
Read noise
45000 e-
Full well capacity
Dynamic range
bit, 12-bit, 11-bit, 8-bit
Bit depth
Binning options
Windows with LAS X
application
Operating system
60 W
Power consumption
Operating temperature
Leica Microsystem I THUNDER Imager 3D Assay & Organoid Imaging | MC--0006930
Each THUNDER Imager Cell can be a high-speed imaging device whose core components are tightly real-time controlled by Synapse.
Synapse synchronizes the camera exposure with the actual illumination time. For this reason, Synapse helps to minimize light exposure, photodamage and bleaching by enabling high-speed acquisition.
Each THUNDER Imager Cell can be equipped with a high-end LED light source, which offers a number of advantages for the user.
0
Image your samples with high-speed, versatile, and gentler LED light sources.
3D cell culture samples are typically grown in hydrogels and are comparably far away from the bottom of the sample carrier.
Long distance water immersion objectives are specifically designed for these types of samples and typically provide much better resolution and higher image brightness as compared to air lenses.
Keep in mind:
Image brightness is proportional to the power of the NA
Matching the RI of specimen and immersion drives image quality and system sensitivity
Reduce Time to Result: High-Speed Positioning with the Quantum Stage
Reduce Time to Result: High Speed, More Data Points, Better Statistics
Reduce Time to Result - Application Workflow Example
More time on the THUNDER Imager to perform experiments
11640708 Processing and Analysis Workstation
Processing and Analysis Workstation includes PC with preinstalled LAS Office and the following LAS X licenses:
3D Visualization Basic
Stitching
Ratio Imaging
Colocalisation
Dye Finder
FRET
11640709 Processing and Analysis Power Workstation
following LAS X licenses:
- 3D Visualization Basic
- Stitching
- Ratio Imaging
- Colocalisation
- Dye Finder
- FRET
Processing and Analysis Power Workstation includes PC with preinstalled LAS X Office and the
Options:
11889226 THUNDER Offline License OPTION
11889226 THUNDER Offline is an optional license package exclusively for the following two workstations:
Processing and Analysis Workstation
Processing and Analysis Power Workstation
Images acquired on a THUNDER Imager with 3D DCV station and LAS X 3.8.0 or higher or Mica system with LAS X 6.2 .0 or higher can be THUNDERed with ICC, SVCC, LVCC.
Images acquired on a THUNDER Imager with 2D station and LAS X 3.8.0 or higher can be THUNDERed with ICC, CC.
Please note: THUNDER Offline only works for images deriving from a THUNDER Imager or Mica system
MICA OPTION
Learn & Results
ADDITIONAL LAS X OPTIONS
2D Analysis
3D Analysis
AIVIA OPTIONS
calling to Aivia options in CPQ
LAS X Office: Learn and Results
Resu
1
.
0 .
LAS X Office: Context Navigator
Promet statinos
Organoid/ Spheroid Core
Fluo turret, mot. 6 pos. with LID
EFW (4 channel)
Motorized 6-pos. objective turret with 4x, 10x, 20x and 40x
Motorized Z-focus drive
Scanning stage
LAS X Navigator
K5 sCMOS camera
LED5 + pod
Aivia Go (1 year)
Application Examples
Case Studies
Image Galleries
Image Gallery: THUNDER Imager T.m.g. posen. cloud the vew of thic sesmples when using camera-base
Webinars
"See the Hidden" On Demand
New e-Book on Organoids & Spheroids!
Delve into case studies including:
-Examining 'Brains-In-A-Dish' from induced
pluripotent stem cells (iPSCs)
Observing 3D cell cultures during development
-Developing heart pacemaker cells from cardiac spheroids
Learn more about:
Key considerations for imaging organoids and spheroids
Solutions for examining dynamic processes in organoids and spheroids in real-time
Going deeper into 3D with correlative light
Biopharma and electron microscopy (CLEM)
Academia
versions available
Additional Resources
Whitepapers
From Eye to Insight
Fluo turret, mot. 6 pos. with LID
EFW (>4+ channels)
Motorized 6-pos. objective turret with 4x, 10x, 20x and 40x