| Basic Information |
| Event |
GTS 40-3-2 |
| OECD UI |
MON-Ø4Ø32-6 |
| Trade Name |
Roundup Ready® Soybean |
| Species |
Glycine max (Soybean) |
| Introduced Trait |
Herbicide Tolerant |
| Transformation Method |
Microparticle bombardment |
| Transformation Vector |
PV-GMGT04, |
| Developer |
Monsanto Company |
|
| Introduced Elements |
| All Known Sequence: |
| (1) |
| ref: | AJ308515 || AJ308514 || AB209952 | |
| Plant DNA |
CaMV 35S |
CTP4 |
Cp4 epsps |
nos |
Cp4 epsps trunc |
unknown |
Plant DNA |
|
| + View
Complete Sequence & Detailed Infomation |
| 1 | CCCAAGTTCC TAAATCTTCA AGTTTTCTTG TTTTTGGATC TAAAAAACTG | | 51 | AAAAATTCAG AACCTTGTGC AAATTATTCA AACCCTTCAA TTTAACCGAT | | 101 | GCTAATGAGT TATTTTTGCA TGCTTTAATT TGTTTCTATC AAATGTTTAT | | 151 | TTTTTTTTAC TAGAAATAAC TTATTGCATT TCATTCAAAA TAAGATCATA | | 201 | CATACAGGTT AAAATAAACA TAGGGAACCC AAATGGAAAA GGAAGGTGGC | | 251 | TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC | | 301 | CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG | | 351 | TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT | | 401 | GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA | | 451 | AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGGACACGCT |
|
|
| Target Gene |
cp4 epsps cassette |
Promotor |
P-35S-CTP4 |
| enhanced CaMV 35S;chloroplast transit peptide from Petunia hybrida |
Coding Region |
Coding 5-enolpyruvylshikimate-3-phosphate synthase |
| From Agrobacterium tumefaciens |
Terminator |
NOS 3' |
| A. tumefaciens nopaline synthase (nos) 3'-polyadenylation signal |
|
| Molecular
Characterization |
|
As mentioned in the Monsanto's original petition, GTS40-3-2 contains a single insert, containing P-35S, CTP4, cp4 epsps, and T-nos. No other elements are found in the cotton genome.
Additional studies conducted by Monsanto showed that the insert of T-nos is complete. A 250 bp segment of the cp4 epsps, and a 534 bp rearrangement of the DNA are found adjacent to the 3' end of the T-nos. Besides, a 72 bp cp4 epsps inactive fragment is also found in the genome.
|
| Nucleic Acid - Based Method |
| For Exogenous Gene: |
|
|
| *View All Possible
Screen Method |
| *View All
Possible Gene-Specific Method |
| For Endogenous Reference Gene: |
|
|
lectin |
Acc. No： |
K00821 |
Copy No (haploid): |
1 |
|
| Protein - Based Method |
| Reported Method |
|
|
| Commercial Kit |
|
|
|
| Certified Reference Materials |
|
|
| *View All CRMs (The CRMs are provided by
IRMM, AOCS, and Sigma) |
| Reference Molecules: pMuISL2,
pMuISL2,
|
| View
other Information on Agbios... |
