Liquiritin Carbomer Gel Cold Paste Promotes Healing of Solar Dermatitis in Mice 甘草苷聚卡波姆凝胶冷敷贴促进小鼠太阳皮炎的愈合
Yanfang Huang , Sijia , Jinghua Pan , Congjing Song , Weiqiang Chen and Yun Zhang Yanfang Huang ,Sijia ,Jinghua Pan ,Congjing Song ,Weiqiang Chen 和 Yun Zhang 1 School of Nursing, Guangdong Pharmaceutical University, Guangzhou 510006, China 广东药科大学护理学院,中国广州 5100062 School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China 广东药科大学药学院,中国广州 5100063 School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China 广东药科大学基础医学院,中国广州 510006* Correspondence: wqc@gdpu.edu.cn (W.C.); zhangyun@gdpu.edu.cn (Y.Z.) * 通讯:wqc@gdpu.edu.cn(W.C.); zhangyun@gdpu.edu.cn(Y.Z.)+ These authors contributed equally to this work. 这些作者对这项工作做出了相等的贡献。
Citation: Huang, Y.; Li, S.; Pan, J.; Song, C.; Chen, W.; Zhang, Y. Liquiritin Carbomer Gel Cold Paste Promotes Healing of Solar Dermatitis in Mice. Int. J. Mol. Sci. 2024, 25, 3767. https://doi.org/10.3390/ijms25073767 引用:Huang,Y.;李,S.;潘,J.;宋,C.;陈,W.;张,Y.。甘草素卡波姆凝胶冷膏促进小鼠太阳皮炎愈合。国际分子科学杂志。2024 年,25 卷,3767 页。https://doi.org/10.3390/ijms25073767
Academic Editor: Christopher Jackson 学术编辑:克里斯托弗·杰克逊
Ultraviolet radiation (UVR) has various effects on human cells and tissues, which can lead to a variety of skin diseases and cause inconvenience to people's lives. Among them, solar dermatitis is one of the important risk factors for malignant melanoma, so prevention and treatment of solar dermatitis is very necessary. Additionally, liquiritin (LQ) has anti-inflammatory effects. In this study, we aimed to evaluate the anti-inflammatory and pro-wound healing effects of liquiritin carbomer gel cold paste (LQ-CG-CP) in vitro and in vivo. The results of MTT experiments showed no cytotoxicity of LQ at concentrations of and below and cell damage at UVB irradiation doses above . Moreover, LQ can promote cell migration. ELISA results also showed that LQ inhibited the elevation of the inflammatory factors tumor necrosis factor- (TNF- ), interleukin (IL-1 ), and interleukin-6 (IL-6) after UVB irradiation. In the mouse model of solar dermatitis, LQ-CG-CP showed the best therapeutic efficacy for wound healing and relief of itching compared to MEIBAO moist burn moisturizer (MEBO). What is more, the results of skin histopathological examination show that LQ-CG-CP promotes re-epithelialization, shrinks wounds, and promotes collagen production, thus promoting wound healing. Simultaneously, LQ-CG-CP reduced TNF- , IL-1 , and IL-6 expression. In addition, LQ-CG-CP was not observed to cause histopathological changes and blood biochemical abnormalities in mice. Overall, LQ-CG-CP has great potential for the treatment of solar dermatitis. 紫外线辐射(UVR)对人体细胞和组织有各种影响,可能导致多种皮肤疾病,并给人们的生活带来不便。其中,太阳性皮炎是恶性黑色素瘤的重要危险因素之一,因此预防和治疗太阳性皮炎非常必要。此外,甘草素(LQ)具有抗炎作用。在本研究中,我们旨在评估离体和体内甘草素羟丙基甲基纤维素凝胶冷贴(LQ-CG-CP)的抗炎和促伤口愈合效果。MTT 实验结果显示,LQ 在 及以下浓度下无细胞毒性,并且在 UVB 照射剂量超过 时造成细胞损伤。此外,LQ 可以促进细胞迁移。ELISA 结果还显示,在 UVB 照射后,LQ 抑制了炎症因子肿瘤坏死因子- (TNF- )、白细胞介素 (IL-1 )和白细胞介素-6(IL-6)的升高。在太阳性皮炎小鼠模型中,与 MEIBAO 湿烧伤保湿剂(MEBO)相比,LQ-CG-CP 对于伤口愈合和缓解瘙痒效果最好。 此外,皮肤组织病理学检查结果显示,LQ-CG-CP 促进了重新上皮化,收缩伤口,并促进了胶原蛋白的产生,从而促进了伤口愈合。同时,LQ-CG-CP 降低了 TNF-7,IL-1 和 IL-6 的表达。此外,在小鼠中未观察到 LQ-CG-CP 引起的组织病理学变化和血液生化异常。总的来说,LQ-CG-CP 在太阳性皮炎治疗方面具有巨大潜力。
Keywords: solar dermatitis; liquiritin carbomer gel cold paste; anti-inflammatory; wound healing 关键词:太阳皮炎;甘草苷卡波姆明胶冷敷膏;抗炎;伤口愈合
1. Introduction 1. 介绍
Nowadays, UVR has become one of the most important factors affecting people's lives due to its various effects on human skin tissue. Specifically, solar dermatitis, also known as sunburn, is an inflammation of the skin caused by exposure to excessive UVR in a sun-exposed environment, which usually leads to the appearance of erythematous pimples and blisters in sun-exposed areas, and self-consciousness of burning, itching, and pain [1]. The shoulders, neck, head, and face are the most common areas of sunburn, followed by the hands or arms and back [2]. The symptoms of solar dermatitis can bring inconvenience to patients' lives. A study analyzing data from a national sample of U.S. hospital emergency department visits in 2013 estimated 33,826 sunburn-related visits and an estimated USD 11.2 million in costs associated with emergency department visits [3]. A history of solar dermatitis is an important risk factor for malignant melanoma [4]. Therefore, the prevention and treatment of solar dermatitis is essential. 如今,紫外线辐射已成为影响人们生活的最重要因素之一,因为它对人体皮肤组织有各种影响。具体而言,太阳性皮炎,又称晒伤,是皮肤由于在阳光照射环境中暴露于过量紫外线辐射而引起的炎症,通常会导致暴露于阳光下的区域出现红斑丘疹和水疱,并伴有烧灼感、瘙痒和疼痛的自觉[1]。肩部、颈部、头部和面部是最常见的晒伤部位,其次是手部或臂部和背部[2]。太阳性皮炎的症状会给患者的生活带来不便。一项分析 2013 年美国全国样本医院急诊科就诊数据的研究估计有 33,826 次与晒伤相关的就诊,急诊科就诊相关成本估计为 1,120 万美元[3]。太阳性皮炎的病史是恶性黑素瘤的一个重要危险因素[4]。因此,预防和治疗太阳性皮炎至关重要。
UVR is categorized into three types based on their wavelengths: UVA (315-400 nm), UVB (280-315 nm), and UVC (100-280 nm); the atmosphere filters out most of the UVB and all of the UVC, so that the UVA and a portion of the UVB that reaches the Earth can cause damage to the human skin [5]. However, both act differently on the skin and only UVB induces characteristic epidermal sunburn damage [6]. UVB can induce an 紫外线根据其波长分为三种类型:UVA(315-400 纳米),UVB(280-315 纳米)和 UVC(100-280 纳米); 大气层会过滤掉大部分 UVB 和所有 UVC,使得到达地球的 UVA 和部分 UVB 可以对人体皮肤造成损害[5]。然而,它们在皮肤上的作用不同,只有 UVB 引起特征性的表皮日晒损伤[6]。UVB 能够诱导
inflammatory response through several mechanisms . In addition, UV can directly activate keratinocytes and other cells to release inflammatory mediators such as TNF- , IL-1 , and IL-6 [9]. It has been shown that naringenin can inhibit skin edema induced by UVB irradiation, and also inhibit UVB irradiation-induced MMP-9 activity and the production of inflammatory factors to prevent UVB irradiation damage to mouse skin [10]. Verbenacea extract also had a protective effect on UVB-irradiated mice, inhibiting UVBinduced inflammatory responses as well as oxidative stress [11]. 炎症反应会通过几种机制 。此外,紫外线可以直接激活角质细胞和其他细胞释放炎症介质,如 TNF- ,IL-1 和 IL-6 [9]。已经证明柚皮素可以抑制 UVB 照射引起的皮肤水肿,并抑制 UVB 照射诱导的 MMP-9 活性和炎症因子的产生,以预防 UVB 照射对小鼠皮肤的损伤[10]。马鞭草提取物也对 UVB 照射的小鼠具有保护作用,抑制 UVB 诱导的炎症反应和氧化应激[11]。
In recent years, the role of licorice flavonoids has attracted the attention of some researchers, and their potential medicinal value and application areas have been continuously studied, and their newly developed products have better application prospects [12,13]. LQ is a licorice flavonoid compound extracted from licorice [14]. Its anti-inflammatory effects have been demonstrated in studies of diseases such as rheumatoid arthritis and lipopolysaccharide-induced acute lung injury . 近年来,甘草黄酮的作用引起了一些研究者的注意,其潜在药用价值和应用领域得到了持续研究,并且其新开发的产品具有更好的应用前景【12,13】。LQ 是从甘草中提取的甘草黄酮化合物【14】。研究表明,其具有抗炎作用,可用于类风湿性关节炎和脂多糖诱导的急性肺损伤等疾病的研究 。
Currently, the main research focus is on the protective and preventive effects of solar dermatitis, while this paper focuses on the therapeutic perspective. The carbomer gel selected in this study can prolong the retention time of the drug on the skin surface, improve the efficiency of drug use, and, on this basis, when combined with a cold compress paste, can soothe the burning, itching, and other discomforts caused by solar dermatitis. Therefore, this study proposed to study the therapeutic effect of LQ-CG-CP in promoting wound healing of solar dermatitis in mice and explore the mechanism of its treatment of solar dermatitis, to provide a certain research basis for the subsequent development of the cold paste of solar dermatitis therapeutic gel, which has a certain clinical application prospect. 目前,主要的研究重点是太阳皮炎的保护和预防效果,而本文则侧重于治疗角度。本研究选择的卡波姆凝胶可以延长药物在皮肤表面的停留时间,提高药物使用效率,在此基础上,与冷敷膏结合时,可以缓解太阳皮炎引起的灼烧、瘙痒和其他不适感。因此,本研究提出了研究 LQ-CG-CP 促进小鼠太阳皮炎伤口愈合的治疗效果,并探索其治疗太阳皮炎的机制,为后续太阳皮炎治疗凝胶冷敷膏的开发提供一定的研究基础,具有一定的临床应用前景。
2. Results 2. 结果
2.1. Effect of LQ and UVB on Cell Proliferation Viability 2.1. LQ 和 UVB 对细胞增殖活力的影响
To confirm the concentration of LQ used and the UVB modeling dose, the MTT assay was used to detect the effects of LQ and UVB on HaCaT and JB6 cells. As shown in Figure 1A,B, the viability of HaCaT and JB6 cells was significantly decreased at concentrations of LQ of or more, indicating that high concentrations of LQ were significantly toxic to the cells . Therefore, of LQ was used as the highest concentration for subsequent experiments. The survival rates of and JB6 cells were significantly decreased when UVB was at a dose of or above (Figure 1C,D). Compared with the blank control group, the cell survival rate in the dose group was higher than that in the 70 and dose groups, although it decreased; so, the dose of UVB was the optimal dose for the cell model. 为了确认所使用的 LQ 浓度和 UVB 模拟剂量,使用 MTT 测定法检测 LQ 和 UVB 对 HaCaT 和 JB6 细胞的影响。如图 1A、B 所示,HaCaT 和 JB6 细胞的存活率在 LQ 浓度为 或更高时显著降低,表明高浓度的 LQ 对细胞有明显毒性 。因此,将 LQ 的 用作后续实验的最高浓度。当 UVB 剂量为 或以上时, 和 JB6 细胞的存活率显著降低 (图 1C、D)。与空白对照组相比, 剂量组的细胞存活率高于 70 和 剂量组,尽管有所下降;因此,UVB 的 剂量是细胞模型的最佳剂量。
2.2. Effect of LQ on Cell Migration Capacity and UVB on Cell Secretion of Inflammatory Factors 2.2. LQ 对细胞迁移能力和 UVB 对细胞分泌炎症因子的影响
To assess whether LQ could promote cell migration, the cell scratch assay was used to detect the effects of different concentrations of on the migration rate of and JB6 cells. As shown in Figure 2A-D, LQ significantly increased HaCaT and JB6 cell scratch wound migration width . It is thus clear that LQ promotes and JB6 cell migration. 评估 LQ 是否能促进细胞迁移,使用细胞划痕实验检测不同浓度的 对 和 JB6 细胞迁移速率的影响。如图 2A-D 所示,LQ 显著增加了 HaCaT 和 JB6 细胞划痕伤口迁移宽度 。因此清楚地表明 LQ 促进了 和 JB6 细胞迁移。
To determine the effect of UVB on cell secretion of inflammatory factors, the ELISA assay was used to detect the effects of different UVB irradiation doses on the expression levels of cellular inflammatory factors. As shown in Figure 2E-J, the higher the UVB modeling dose, the higher the levels of TNF- , IL-1 , and IL-6. 为了确定 UVB 对细胞分泌炎症因子的影响,使用 ELISA 检测了不同 UVB 照射剂量对细胞炎症因子表达水平的影响。如图 2E-J 所示,UVB 建模剂量越高,TNF-α、IL-1β和 IL-6 水平越高。
2.3. Effect of LQ on Cell Secretion of Inflammatory Factors after UVB Irradiation 2.3. LQ 对 UVB 照射后细胞分泌炎症因子的影响
To determine whether LQ could inhibit the elevation of inflammatory factors in and JB6 cells induced by UVB irradiation, ELISA experiments were used to detect the effects of different LQ concentrations on the expression levels of inflammatory factors in and JB6 cells after UVB irradiation. As shown in Figure 3, the UVB irradiation dose significantly increased the expression levels of the inflammatory factors TNF- , IL-1 , and IL-6 in HaCaT and JB6 cells compared with the Control group ). In HaCaT cells, 确定 LQ 是否能抑制 UVB 照射诱导的 和 JB6 细胞中炎症因子的升高,ELISA 实验用于检测不同 LQ 浓度对 UVB 照射后 和 JB6 细胞中炎症因子表达水平的影响。如图 3 所示,与对照组相比( ), HaCaT 和 JB6 细胞中的 UVB 照射剂量显著增加了炎症因子 TNF- ,IL-1 和 IL-6 的表达水平。在 HaCaT 细胞中,
the administration of and LQ interventions significantly decreased the levels of TNF- , IL-1 , and IL-6 and alleviated the inflammatory response in a dose-dependent manner (Figure 3A-C). In JB6 cells, the administration of and LQ interventions significantly decreased the level of TNF- (Figure 3D); the administration of LQ interventions significantly decreased the level of IL-1 ) (Figure 3E); and the administration of LQ interventions failed to significantly decrease the level of IL-6, but it also followed an increase in dose and showed a decreasing trend ) (Figure 3F). 和 LQ 干预的管理显著降低了 TNF- ,IL-1 和 IL-6 的水平,并以剂量依赖的方式缓解了炎症反应(图 3A-C)。在 JB6 细胞中, 和 LQ 干预的管理显著降低了 TNF- 的水平(图 3D); LQ 干预的管理显著降低了 IL-1 的水平(图 3E);LQ 干预的管理未能显著降低 IL-6 的水平,但随着剂量的增加也呈现出下降的趋势 (图 3F)。
A
C
B
D
Figure 1. Effect of LQ and UVB on cell viability. (A) Effect of LQ on HaCaT cell viability; (B) Effect of LQ on JB6 cell viability; (C) Effect of UVB on HaCaT cell viability; and (D) Effect of UVB on JB6 cell viability. (ns ). 图 1. LQ 和 UVB 对细胞存活率的影响。(A) LQ 对 HaCaT 细胞存活率的影响;(B) LQ 对 JB6 细胞存活率的影响;(C) UVB 对 HaCaT 细胞存活率的影响;以及(D) UVB 对 JB6 细胞存活率的影响。(ns )。
2.4. The Promoting Effect of LQ on Skin Wound Healing and Its Ability to Alleviate Itching Symptoms in Mice 2.4. LQ 对小鼠皮肤伤口愈合的促进作用及其缓解瘙痒症状的能力
As shown in Figure 4A, which shows the process of skin wound changes in each group, the Control group is the normal skin of mice. Compared with the UVB group, the wound size was significantly smaller in the LQ-CG-CP group and the LQ-CG-CP group, which could also be found to be more effective than the application of CP and LQ-CG alone. In addition, the effect of the positive drug MEBO was not as significant as that of LQ-CG-CP. As shown in Figure 4B, after 7 days of treatment, the average wound healing rates of mice in the UVB group, CP group, LQ-CG group, LQ-CG-CP group, 1% LQ-CG-CP group, 2% LQ-CG-CP group, and MEBO group were , , and . Compared with the UVB group, the wound healing rates of mice in the CP, LQ-CG, LQ-CG-CP, LQ-CG-CP, LQ-CG-CP, and MEBO groups were significantly increased , with the most significant healing effect in the LQ-CG-CP and LQ-CG-CP groups. What is more, mice with solar dermatitis develop itching symptoms, and the severity of itchy skin symptoms in mice was visually evaluated by observing the number of scratches. As shown in Figure 4C, compared with the UVB group, the itching number of mice in the CP group decreased slightly, while the itching behaviors of mice in the LQ-CG group, the LQ-CG-CP group, the LQ-CG-CP group, the LQ-CG-CP group, and the MEBO group could be 如图 4A 图 4A 所示,展示了每组皮肤伤口变化的过程,对照组为小鼠正常皮肤。与 UVB 组相比,0 LQ-CG-CP 组和 1 LQ-CG-CP 组的伤口大小显著较小,这也比单独应用 CP 和 2 LQ-CG 更有效。此外,正性药物 MEBO 的效果并不如 LQ-CG-CP 显著。如图 4B 所示,在治疗 7 天后,UVB 组、CP 组、3 LQ-CG 组、4 LQ-CG-CP 组、1% LQ-CG-CP 组、2% LQ-CG-CP 组和 MEBO 组小鼠的平均伤口愈合率分别为 5、6 和 7。与 UVB 组相比,CP 组、8 LQ-CG 组、9 LQ-CG-CP 组、10 LQ-CG-CP 组、11 LQ-CG-CP 组和 MEBO 组小鼠的伤口愈合率显著增加,其中 ,在 14 LQ-CG-CP 组中愈合效果最为显著。此外,太阳性皮炎小鼠出现瘙痒症状,通过观察抓挠次数对小鼠的瘙痒皮肤症状 LQ-CG-CP 组具有最显著的愈合效果。此外,太阳皮炎小鼠会出现瘙痒症状,通过观察划痕数量对小鼠瘙痒皮肤症状的严重程度进行视觉评估。如图 4C 所示,与 UVB 组相比,CP 组小鼠的瘙痒次数略有减少,而 15 LQ-CG 组、16 LQ-CG-CP 组、17 LQ-CG-CP 组、18 LQ-CG-CP 组和 MEBO 组小鼠的瘙痒行为可能会稍有
significantly reduced ( , with the greatest decrease in the number of scratches of mice in the LQ-CG-CP group ( ), indicating the best effect. 显著减少( ,老鼠在 LQ-CG-CP 组中划痕数量的减少最大( ),表明效果最佳。
Figure 2. Effects of LQ and UVB on HaCaT and JB6 cells. (A) The change process of HaCaT cell scratch; (B) The scratch wound closure rate of HaCaT cells; (C) The change process of JB6 cell scratch; (D) The scratch wound closure rate of JB6 cells; (E-G) UVB-induced expression levels of inflammatory factors TNF- , IL-1 , and IL-6 in HaCaT cells; and (H-J) UVB-induced expression levels of inflammatory factors TNF- , IL-1 , and IL-6 in JB6 cells. (ns , , magnifications, ). 图 2. LQ 和 UVB 对 HaCaT 和 JB6 细胞的影响。 (A) HaCaT 细胞划痕的变化过程;(B) HaCaT 细胞划痕愈合速率;(C) JB6 细胞划痕的变化过程;(D) JB6 细胞划痕愈合速率;(E-G) HaCaT 细胞中 UVB 诱导的炎症因子 TNF- ,IL-1 和 IL-6 的表达水平;(H-J) JB6 细胞中 UVB 诱导的炎症因子 TNF- ,IL-1 和 IL-6 的表达水平。 (ns , ,放大倍数, )。
2.5. Staining Results of Wound Skin Tissues 2.5. 伤口皮肤组织的染色结果
To study the process of LQ-CG-CP in promoting wound healing, mouse skin tissues were taken for and Masson staining to assess the rate of wound repair in mouse skin tissues after 7 days of administration. 研究 LQ-CG-CP 在促进伤口愈合过程中的作用,取得了小鼠皮肤组织用于 和 Masson 染色,以评估给药后小鼠皮肤组织在 7 天后的伤口修复速率。
The HE staining results are shown in Figure 5A. Some areas in the UVB model group were not completely healed, did not possess complete epidermis, and still had inflammatory cell infiltration. There was a significant difference in epidermal thickness in the UVB, CP, LQ-CG, LQ-CG-CP, LQ-CG-CP, and MEBO groups compared to the Control HE 染色结果如图 5A 所示。在 UVB 模型组中,一些区域并未完全愈合,未具有完整的表皮,仍然存在炎性细胞浸润。与对照组相比,UVB、CP、 LQ-CG、 LQ-CG-CP、 LQ-CG-CP 和 MEBO 组的表皮厚度存在显著差异。
group ( ). Compared with the UVB group, the epidermal thickness of the CP, LQCG, LQ-CG-CP, LQ-CG-CP, 2% LQ-CG-CP, and MEBO groups was significantly reduced (Figure 5B). In contrast, LQ-CG-CP had formed a complete epidermis, which was close to the skin histological structure of the blank control group. The epidermis of the MEBO group was thickened, the epidermis was still not completely healed, and the stratum corneum was disorganized. 组( )。与 UVB 组相比,CP, LQCG, LQ-CG-CP, LQ-CG-CP,2% LQ-CG-CP 和 MEBO 组的表皮厚度显著减少 (图 5B)。相反, LQ-CG-CP 形成了完整的表皮,接近空白对照组的皮肤组织结构。 MEBO 组的表皮变厚,表皮仍未完全愈合,角质层无序。
A
B
C
Figure 3. Effect of LQ on the expression levels of inflammatory factors in UVB-induced and JB6 cells. (A-C) The levels of TNF- , IL-1 , and IL-6 in HaCaT cells; and (D-F) The levels of TNF- , IL-1 , and IL-6 in JB6 cells. ( ; +: Positive, - : negative). 图 3。LQ 对 UVB 诱导的 HaCaT 和 JB6 细胞中炎症因子表达水平的影响。(A-C)HaCaT 细胞中 TNF-α、IL-1β 和 IL-6 的水平;以及(D-F)JB6 细胞中 TNF-α、IL-1β 和 IL-6 的水平。(+:阳性,-:阴性)。
The collagen fibers of the skin tissue were blue after MT staining. As shown in Figure 5C, which shows the collagen deposition in each subgroup after 7 days of treatment, compared with the UVB and CP groups, the collagen fibers in the LQ-CG-CP and LQ-CG-CP groups were in a tightly ordered arrangement, with smaller epithelial tissue gaps, which was similar to the normal skin tissue structure in the Control group. Although a large number of collagen fibers were also formed in the MEBO group, their arrangement and distribution were more disorderly. In Figure 5D, it is shown that collagen deposition was significantly increased in the LQ-CG-CP, LQ-CG-CP, and MEBO groups compared to the UVB group ( ). 皮肤组织的胶原纤维在 MT 染色后呈蓝色。如图 5C 所示,显示了治疗 7 天后每个亚组中的胶原沉积情况,与 UVB 和 CP 组相比, LQ-CG-CP 和 LQ-CG-CP 组的胶原纤维排列紧密有序,上皮组织间隙较小,与对照组的正常皮肤组织结构相似。尽管 MEBO 组也形成了大量胶原纤维,但它们的排列和分布更加混乱。在图 5D 中,显示了与 UVB 组相比, LQ-CG-CP, LQ-CG-CP 和 MEBO 组的胶原沉积显着增加( )。
2.6. Effect of LQ-CG-CP on Inflammatory Factors in a Mouse Model of Solar Dermatitis 2.6. LQ-CG-CP 对太阳皮炎小鼠模型炎症因子的影响
ELISA experiments were used to detect the effects of different LQ concentrations on the expression levels of inflammatory factors in a mouse model of solar dermatitis. As shown in Figure 6, the expression levels of inflammatory factors TNF- , IL-1 , and IL-6 were significantly increased in the UVB modeling group of mice compared with the blank control group . After 7 days of administration, the CP group could not significantly decrease the expression levels of TNF- , IL-1 , and IL-6, while the administration groups showed a significant decrease ) in the expression levels of TNF- , IL-1 , and IL-6 and alleviated the inflammatory response in a dose-dependent manner. In addition, the expression levels of IL-1 and IL-6 were lower in the LQ-CG-CP group than in the positive drug MEBO group. ELISA 实验用于检测不同 LQ 浓度对太阳皮炎小鼠模型炎症因子表达水平的影响。如图 6 所示,与空白对照组相比,UVB 模型组小鼠的炎症因子 TNF- 、IL-1 和 IL-6 的表达水平显著增加 。给药 7 天后,CP 组无法显著降低 TNF- 、IL-1 和 IL-6 的表达水平,而给药组显示出明显降低 )TNF- 、IL-1 和 IL-6 的表达水平,并且呈剂量依赖性减轻炎症反应。此外,LQ-CG-CP 组中 IL-1 和 IL-6 的表达水平低于阳性药物 MEBO 组。
B
Figure 4. The characterization results of skin tissues during the treatment process. (A) Diagram of the skin wound healing process in each group of mice (scale bar, ); (B) Skin wound healing rate in each group of mice after 7 days of treatment; and (C) Pruritus situation in mice after 7 days of treatment. ( ). 图 4. 治疗过程中皮肤组织的表征结果。 (A)小鼠各组皮肤伤口愈合过程示意图(比例尺, );(B)治疗 7 天后各组小鼠皮肤伤口愈合率;以及(C)治疗 7 天后小鼠的瘙痒情况。 ( )。
A
C
B
Figure 5. Results of HE and MT staining of skin wounds of mice in each group after 7 days of administration (100 ). (A) HE staining; (B) Changes in epidermal thickness of the skin; (C) MT staining; and (D) Quantification of skin collagen deposition. ( , ,