LOCUS       X01714                  1609 bp    DNA     linear   BCT 23-OCT-2008
DEFINITION  E. coli dut gene for dUTPase (EC 3.6.1.23) (deoxyuridine
            5'-triphosphate nucleotidohydrolase).
ACCESSION   X01714
VERSION     X01714.1
KEYWORDS    dUTPase; unidentified reading frame.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Lundberg,L.G., Thoresson,H.O., Karlstrom,O.H. and Nyman,P.O.
  TITLE     Nucleotide sequence of the structural gene for dUTPase of
            Escherichia coli K-12
  JOURNAL   EMBO J. 2 (6), 967-971 (1983)
   PUBMED   6139280
COMMENT     Data kindly reviewed (25-NOV-1985) by L. Lundberg.
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     regulatory      286..291
                     /regulatory_class="promoter"
                     /note="-35 region"
     regulatory      310..316
                     /regulatory_class="promoter"
                     /note="-10 region"
     misc_feature    322..324
                     /note="put. transcription start region"
     regulatory      330..333
                     /regulatory_class="ribosome_binding_site"
                     /note="put. rRNA binding site"
     CDS             343..798
                     /note="unnamed protein product; dUTP-ase (aa 1-151)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA25859.1"
                     /db_xref="GOA:P06968"
                     /db_xref="InterPro:IPR008180"
                     /db_xref="InterPro:IPR008181"
                     /db_xref="PDB:1DUD"
                     /db_xref="PDB:1DUP"
                     /db_xref="PDB:1EU5"
                     /db_xref="PDB:1EUW"
                     /db_xref="PDB:1RN8"
                     /db_xref="PDB:1RNJ"
                     /db_xref="PDB:1SEH"
                     /db_xref="PDB:1SYL"
                     /db_xref="PDB:2HR6"
                     /db_xref="PDB:2HRM"
                     /db_xref="UniProtKB/Swiss-Prot:P06968"
                     /translation="MKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLNDAVELAP
                     GDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWN
                     RGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     misc_feature    831..851
                     /note="put.stem-loop structure"
     repeat_region   831..838
                     /note="inverted repeat A"
     repeat_region   844..851
                     /note="inverted repeat A'"
     misc_feature    866..893
                     /note="put. stem-loop structure"
     repeat_region   866..872
                     /note="imp. inverted repeat B"
     repeat_region   888..893
                     /note="imp. inverted repeat B'"
     regulatory      889..895
                     /regulatory_class="ribosome_binding_site"
                     /note="pot. rRNA binding site"
     CDS             905..1540
                     /note="unnamed protein product; unidentified reading
                     frame"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA25860.1"
                     /db_xref="GOA:P0C093"
                     /db_xref="InterPro:IPR001647"
                     /db_xref="InterPro:IPR009057"
                     /db_xref="InterPro:IPR011075"
                     /db_xref="InterPro:IPR015893"
                     /db_xref="UniProtKB/Swiss-Prot:P0C093"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAASCSNMTPDDFSSGEFL"
ORIGIN      
        1 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
       61 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
      121 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
      181 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
      241 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
      301 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
      361 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
      421 tctgccggac ttgacctgcg tgcctgtctc aacgacgccg tagaactggc tccgggtgac
      481 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
      541 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
      601 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
      661 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
      721 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
      781 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
      841 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
      901 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
      961 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     1021 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     1081 cgatagcctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     1141 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     1201 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     1261 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     1321 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     1381 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     1441 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgcggcca gttgcagtaa
     1501 tatgacgccg gatgactttt catccggcga gtttctttaa acgccaaact cttcgcgata
     1561 ggccttaacc gccgccagat gttccgccat ttccggcttc tcttccagg
//
位点 X01714 1609 bp DNA 线性 BCT 2008 年 10 月 23 日 定义 dUTP 酶 （EC 3.6.1.23） 的大肠杆菌 dut 基因（脱氧尿苷 5'-三磷酸核胫糖水解酶）。ACCESSION X01714 VERSION X01714.1 关键词 dUTPase;身份不明的阅读框架。来源：大肠杆菌 生物体 大肠杆菌细菌;假单胞菌;伽马变形菌;肠杆菌;肠杆菌科;埃塞里奇亚。参考文献 1（碱基 1 至 1609） 作者 Lundberg，L.G.， Thoresson，H.O.， Karlstrom，O.H. and Nyman，P.O. TITLE： NUCLEOTIDE SEQUENCE OF THE STRUCTURAL GENE FOR DUTPase for dUTPase of Escherichia coli K-12 JOURNAL EMBO J. 2 （6）， 967-971 （1983） PUBMED 6139280 评论 数据由 L. Lundberg （25-NOV-1985） 审阅。特点 位置/限定符 来源 1..1609
/organism=“大肠杆菌”
/mol_type=“基因组DNA”
/db_xref=“分类群：562”
监管 286..291
/regulatory_class=“发起人”
/note=“-35 区域”
监管 310..316
/regulatory_class=“发起人”
/note=“-10 区域”
misc_feature 322..324
/note=“放。转录起始区域”
监管 330..333
/regulatory_class=“ribosome_binding_site”
/note=“放。rRNA结合位点”
CDS 343..798
/note=“未命名的蛋白质产物;dUTP-ase（aa 1-151）”
/codon_start=1
/transl_table=11
/protein_id=“CAA25859.1”
/db_xref=“GOA：P06968”
/db_xref=“InterPro：IPR008180”
/db_xref=“InterPro：IPR008181”
/db_xref=“PDB：1DUD”
/db_xref=“PDB：1DUP”
/db_xref=“PDB：1EU5”
/db_xref=“PDB：1EUW”
/db_xref=“PDB：1RN8”
/db_xref=“PDB：1RNJ”
/db_xref=“PDB：1SEH”
/db_xref=“PDB：1SYL”
/db_xref=“PDB：2HR6”
/db_xref=“PDB：2HRM”
/db_xref=“UniProtKB/Swiss-Prot：P06968”
/translation=“MKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLNDAVELAP
GDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVWN
RGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ”
misc_feature 831..851
/note=“put.stem-loop 结构”
repeat_region 831..838
/note=“倒置重复 A”
repeat_region 844..851
/note=“倒置重复 A'”
misc_feature 866..893
/note=“放。茎环结构”
repeat_region 866..872
/note=“倒置重复 B”
repeat_region 888..893
/note=“倒置重复 B'”
监管 889..895
/regulatory_class=“ribosome_binding_site”
/note=“锅。rRNA结合位点”
CDS 905..1540
/note=“未命名的蛋白质产物;身份不明的读物
框架”
/codon_start=1
/transl_table=11
/protein_id=“CAA25860.1”
/db_xref=“GOA：P0C093”
/db_xref=“InterPro：IPR001647”
/db_xref=“InterPro：IPR009057”
/db_xref=“InterPro：IPR011075”
/db_xref=“InterPro：IPR015893”
/db_xref=“UniProtKB/Swiss-Prot：P0C093”
/translation=“MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLLLGFGERN
PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAASCSNMTPDDFSSGEFL”
原产地 1 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa 61 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat 121 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc 181 ttgatctgat ctgcgcgaac gatgtttccc ag ccaactca aggatttaac agcgacaaca 241 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc 301 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt 361 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc 421tctgccggac ttgacctgcg tgcctgtctc aacgacgccg tagaactggc tccgggtgac 481 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg 541 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga 601 ttgatcgatt ctgactatca gggccagttt g atgatttccg tgtggaaccg tggtcaggac 661 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag 721 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt 781 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat 841ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg 901 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct 961 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc 1021 ctctgtcggc gtttccgaag cggcactgta tcgg ccacttc cccagtaaga ccccgcatgtt 1081 cgatagcctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa 1141 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg 1201 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga 1261tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt 1321 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc 1381 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa 1441 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgcggcca gttgcagtaa 1501 tatgacgccg gatgactttt catccggcgagtttctttaa acgccaaact cttcgcgata 1561 ggccttaacc gccgccagat gttccgccat ttccggcttc tcttccagg //