(10) Patent No.: US 6,369,893 B1 (10) 专利号:US 6,369,893 B1
(45) Date of Patent: (45) 专利日期:
Apr. 9, 2002 四月 9, 2002
(54) MULTI-CHANNEL OPTICAL DETECTION SYSTEM (54) 多通道光学检测系统
(75) Inventors: Lee A. Christel, Palo Alto; M. Allen Northrup, Berkeley; Kurt E. Petersen, San Jose; William A. McMillan, Cupertino; Gregory T. A. Kovacs, Stanford; Steven J. Young, Los Gatos; Ronald Chang, Redwood City; Douglas B. Dority, Mill Valley; Raymond T. Hebert, Los Gatos; Gregory J. Kintz, Mountain View, all of CA (US) (75) 发明者:Lee A. Christel,帕洛阿尔托; M. Allen Northrup,伯克利; Kurt E. Petersen,圣何塞; William A. McMillan,库比蒂诺; 斯坦福大学的 Gregory T. A. Kovacs; Steven J. Young,洛斯加托斯; Ronald Chang,红木城; Douglas B. Dority,米尔谷; Raymond T. Hebert,洛斯加托斯; Gregory J. Kintz,山景城,加利福尼亚州(美国)
(73) Assignee: Cepheid, Sunnyvale, CA (US) (73) 任务分配人:Cepheid, Sunnyvale, CA (US)
(*) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 0 days. (*) 注意:根据任何免责声明,本专利的期限根据 35 U.S.C. 154(b) 延长或调整 0 天。
Wittwer, C.T. et al. The Light Cycler ^(TM){ }^{\mathrm{TM}} : A Microvolume Multisample Fluorimeter with Rapid Temperature Control, BioTechniques 22:176-181; Jan. 1997. Wittwer, C.T. 等人。 光循环仪 ^(TM){ }^{\mathrm{TM}} :具有快速温度控制的微量多样品荧光计,BioTechniques 22:176-181; 1997 年 1 月。
Primary Examiner-F. L. Evans 主要审查员-F. L. 埃文斯
(74) Attorney, Agent, or Firm-Heller Ehrman White & McAuliffe LLP (74) 律师、代理人或公司-Heller Ehrman White & McAuliffe LLP
ABSTRACT 抽象
An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel [2] having a chamber [10] for holding the mixture. The apparatus also includes a heat-exchanging module [37] having a pair of opposing thermal plates [34A, 34B] for receiving the vessel [2] between them and for heating/and or cooling the mixture contained in the vessel. The module [37] also includes optical excitation and detection assemblies [46,48][46,48] positioned to optically interrogate the mixture. The excitation assembly [46] includes multiple light sources [100] and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly [48] includes multiple detectors [102] and a second set of filters for detecting light emitted from the chamber [10] in multiple emission wavelength ranges. The optics assemblies [46,48][\mathbf{4 6 , 4 8}] thus provide a multi-channel system for detecting a plurality of different target analytes in the mixture. 用于热控制和光学询问反应混合物的装置包括一个容器 [2],该容器具有用于盛放混合物的腔室 [10]。 该设备还包括一个热交换模块[37],该模块具有一对相对的热板[34A,34B],用于接收它们之间的容器[2]以及用于加热/和/或冷却混合物 包含在容器中。 该模块 [37] 还包括光学激发和检测组件 [46,48][46,48] 定位以光学方式询问混合物。 激发组件 [46] 包括多个光源 [100] 和一组滤光片,用于用多个激发光束顺序照亮混合物中标记的分析物 激发波长范围。 检测组件 [48] 包括多个检测器 [102] 和第二组滤光片,用于检测从腔室 [10] 在多个发射波长范围内发射的光。 [46,48][\mathbf{4 6 , 4 8}] 因此,光学组件提供了一个多通道系统,用于检测混合物中的多种不同目标分析物。
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| :---: | :---: | :---: | :---: |
| 5,721,136 A | A | 2/1998 | Finney et al. ........... 435/287.2 |
| 5,783,148 | A | 7/1998 | Cottingham ................ 422/56 |
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| 5,786,182 A | A | 7/1998 | Catanzariti et al. ........ 435/91.1 |
| 5,811,296 | A | 9/1998 | Chemelli et al. ....... 435/287.2 |
| 5,863,790 | A | 1/1999 | Bolea .................... 435/287.4 |
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| 5,958,349 | A | 9/1999 | Petersen et al. ........... 422/198 |
| 6,015,667 | A | 1/2000 | Sharaf ........................ 435/6 |
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| 6,140,054 A | A | 10/2000 | Wittwer et al. ............... 435/6 |
| 6,144,448 A | A | 11/2000 | Mitoma ................... 356/317 |