Peptides are recognized as highly effective and safe bioactive ingredients. However, t their practical application is limited and hampered by harsh conditions for practical drug delivery. Hence, a novel peptide nanocarrier of copper peptide (GHK-Cu) encapsulation developed by liposome technology combined with the classical Chinese concept of rigidity and flexibility. Different polyols were selected as modification ligands for phospholipid bilayers to construct a nano drug-carrying system with high loading rate, good stability and biocompatibility. In vitro, this complex not only significantly retarded the release ability of copper peptides, but also enabled copper peptides to be effectively resistant to enzymatic degradation. Furthermore, cellular experiments showed that this system mainly regulates Nrf2, SIRT1, and PEG2/COX-2-related signaling pathways, thus effectively counteracting cellular inflammation, senescence, and apoptosis from oxidative damage. Interestingly, a green, non-toxic, efficient and convenient antioxidant system was developed for the prevention and deceleration of skin aging.
肽是公认的高效和安全的生物活性成分。然而，它们的实际应用受到限制，并受到实际药物输送的恶劣条件的阻碍。因此，通过脂质体技术结合中国古典的刚性和柔性概念，开发了一种新型铜肽纳米载体 （GHK-Cu） 封装。选择不同的多元醇作为磷脂双层的修饰配体，构建具有高载量率、良好稳定性和生物相容性的纳米载药系统。在体外，该复合物不仅显著延缓了铜肽的释放能力，而且使铜肽能够有效抵抗酶降解。此外，细胞实验表明，该系统主要调节 Nrf2、SIRT1 和 PEG2/COX-2 相关信号通路，从而有效对抗细胞炎症、衰老和氧化损伤引起的细胞凋亡。有趣的是，开发了一种绿色、无毒、高效且方便的抗氧化系统，用于预防和延缓皮肤衰老。